期刊
DEVELOPMENTAL CELL
卷 49, 期 2, 页码 189-+出版社
CELL PRESS
DOI: 10.1016/j.devcel.2019.03.025
关键词
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资金
- Howard Hughes Medical Institute
- National Institutes of Health [GM046383, GM092804, EB002503, GM074827]
- Smith Foundation
- Baxter Foundation
- Helen Hay Whitney Foundation
- Croucher Foundation Joint Universities Summer Teaching Laboratory (JUSTL) program
- Shriners Hospital for Children
- BioX Bowes Fellowship
- Alexander S. Onassis Public Benefit Foundation
- Stanford Cellular and Molecular Biology Training Grant [NIH T32-GM007276]
- Stanford Medical Scientist Training Program [NIH T32-GM007365]
- NIH program project [P50 GM107615]
Efficient chemotaxis requires rapid coordination between different parts of the cell in response to changing directional cues. Here, we investigate the mechanism of front-rear coordination in chemotactic neutrophils. We find that changes in the protrusion rate at the cell front are instantaneously coupled to changes in retraction at the cell rear, while myosin II accumulation at the rear exhibits a reproducible 9-15-s lag. In turning cells, myosin II exhibits dynamic side-to-side relocalization at the cell rear in response to turning of the leading edge and facilitates efficient turning by rapidly re-orienting the rear. These manifestations of front-rear coupling can be explained by a simple quantitative model incorporating reversible actin-myosin interactions with a rearward-flowing actin network. Finally, the system can be tuned by the degree of myosin regulatory light chain (MRLC) phosphorylation, which appears to be set in an optimal range to balance persistence of movement and turning ability.
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