期刊
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
卷 93, 期 -, 页码 58-65出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.dci.2018.12.006
关键词
Goose; MAVS; Newcastle disease virus (NDV); Innate immunity
资金
- National Natural Science Foundation of China [31872453, 31530074]
- National Key Research and Development Program of China [2018YFD0500100]
- Shanghai Key Laboratory of Veterinary Biotechnology [KLAB201702]
Mitochondrial antiviral-signaling protein (MAVS) is an essential adaptor protein in retinoic acid-inducible gene I (RIG-I)-mediated antiviral innate immunity in mammals. In this study, the goose MAVS gene (goMAVS) was identified. The 2019 bp-long goMAVS exhibits 96.2% amino acid similarity compared to the predicted goMAVS. Quantitative real-time polymerase chain reactions showed that goMAVS mRNA was widely expressed in different tissues. The overexpression of goMAVS in goose embryo fibroblast cells up-regulated the mRNA levels of goose interferon-stimulated genes. We concluded that MAVS mediates the activation of type I interferon (IFN) pathway in a species-specific manner. We further demonstrated that a CARD-like domain, transmembrane domain and two previously unidentified domains of goMAVS were essential for the activation of type I IFN pathway. GoMAVS inhibited Newcastle disease virus replication by activating type I IFN pathways, especially at the early stages of infection. Finally, the interaction between goMAVS and goose RIG-I was confirmed. The CARD domain of goMAVS plays a vital role in the interaction. Together, we identified goMAVS as a goRIG-I interactive protein and concluded that goMAVS is involved in the activation of type I IFN pathways in goose cells.
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