期刊
CYTOKINE
卷 116, 期 -, 页码 48-60出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.cyto.2019.01.001
关键词
Retinal pigment epithelial cells; Inflammation; Quercetin; Cytokine
资金
- Ministry of Science and Technology, Taiwan [MOST 101-2320-B-255-003-MY3]
- Chang Gung Medical Research Foundation [CMRPF3E0051, CMRPF3E0052, CMRPF3G0011, CMRPF3G0012, BMRPD16]
- Chang Gung University of Science and Technology [EZRPF3E0601, EZRPF3E0161, EZRPF3E0201, EZRPF3E0191, EZRPF3E0181, EZRPF3E0171, EZRPF3G0551]
Quercetin is a flavonoid polyphenolic compound present in fruits and vegetables that has proven anti-inflammatory activity. The goal of the present investigation was to investigate the effects of quercetin on tumor necrosis factor-alpha (TNF-alpha)-induced inflammatory responses via the expression of ICAM-1 and MMP-9 in human retinal pigment epithelial cells (ARPE-19 cells). Real-time PCR, gelatin zymography, and Western blot analysis showed that TNF-alpha induced the expression of ICAM-1 and MMP-9 protein and mRNA in a time-dependent manner. These effects were attenuated by pretreatment of ARPE-19 cells with quercetin. Quercetin inhibited the TNF-alpha-induced phosphorylation of PKCS, JNK1/2, ERK1/2. Quercetin, rottlerin, SP600125 and U0126 attenuated TNF-alpha-stimulated c-Jun phosphorylation and AP-1 Luc activity. Pretreatment with quercetin, rottlerin, SP600125, or Bay 11-7082 attenuated TNF-alpha-induced NF-kappa B (p65) phosphorylation, translocation and Re1A/p65 Luc activity. TNF-alpha significantly increased MMP-9 promoter activity and THP-1 cell adherence, and these effects were attenuated by pretreatment with quercetin, rottlerin, SP600125, U0126, tanshinone IIA or Bay 117082. These results suggest that quercetin attenuates TNF-alpha-induced ICAM-1 and MMP-9 expression in ARPE-19 cells via the MEK1/2 ERK1/2 and PKC delta JNK1/2 c-Jun or NF-kappa B pathways.
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