期刊
CHEMISTRY-A EUROPEAN JOURNAL
卷 25, 期 20, 页码 5158-5162出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.201900221
关键词
biocatalytic cascade reactions; DNA origami; nanodevices; rotary nanomachines
资金
- Shanghai Sailing Program
- National Natural Science Foundation of China [31600802]
- Shanghai Science and Technology Committee [18DZ1112703]
- Fundamental Research Funds for the Central Universities
Biological macromolecular machines perform impressive mechanical movements. F-adenosine triphosphate (ATP) synthase uses a proton gradient to generate ATP through mechanical rotations. Here, a programmed hexagonal DNA nanomachine, in which a three-armed DNA nanostructure (TAN) can perform stepwise rotations in the confined nanospace powered by DNA fuels, is demonstrated. The movement of TAN can precisely go through a 60 degrees rotation, which is confirmed by atomic force microscopy, and each stepwise directional rotating is monitored by fluorescent measurements. Moreover, the rotary nanomachine is used to spatially organize cascade enzymes: glucose oxidase (GOx) and horseradish peroxidase (HRP) in four different arrangements. The multistep regulations of the biocatalytic activities are achieved by employing TAN rotations. This work presents a new prototype of rotary nanodevice with both angular and directional control, and provides a nanoscale mechanical engineering platform for the reactive molecular components, demonstrating that DNA-based framework may have significant roles in futuristic nanofactory construction.
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