期刊
CHEMBIOCHEM
卷 20, 期 17, 页码 2222-2226出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbic.201900137
关键词
bioorthogonal chemistry; protecting groups; cyclopropene; reductases; tetrazines
We describe a modular activation strategy for cyclopropene-tetrazine ligation. This activation strategy uses chemically diverse enzyme- or photolabile protecting groups as cyclopropene reactivity cages. The linkages between the caging groups and cyclopropene are through carbamates, thus permitting the application of diverse cages to allow bioorthogonal reactivity by administering enzymes or light.
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