期刊
CARBOHYDRATE POLYMERS
卷 212, 期 -, 页码 387-394出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2019.02.061
关键词
Chondroitin sulfate; Magnesium; Anti-inflammatory; Apoptosis
资金
- National Foundation of Science and Technology [11872200]
- Guangdong Foundation of Science and Technology [2017B030301018]
- Shenzhen Science and Technology Innovation Committee [JCYJ20160517160827379, JCYJ20170817111312887, GRCK20160829195523424, ZDSYS2014 0509142721429]
- Guangzhou Scientific and Technique Department [201704020129, 201704020069]
Magnesium chondroitin sulfate (MgCS) has been fabricated and characterized in this study. We investigated its morphology, composition as well as structure. The results verify that the sodium of sodium chondroitin sulfate (CS) is successfully replaced by magnesium and formed a polysaccharide-metal ion complex. To evaluate the clinical potential of MgCS, cell proliferation and apoptosis test were conducted. The results reveal that MgCS could effectively increase the proliferation and decrease the apoptosis of osteoarthritis (OA) chondrocytes. Moreover, real-time quantitative polymerase chain reaction (RT-qPCR) was conducted to evaluate the gene expression level. RT-qPCR analysis suggests that MgCS could significantly increase the expression of COLII and decrease the expression of IL-1 beta and iNOS in OA chondrocytes. Furthermore, significant upregulation of Bcl-2 mRNA expression and downregulation of the expression of apoptosis related gene p53 were observed. Thus, it is indicated that MgCS should have great potentials in OA treatment.
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