期刊
CARBOHYDRATE POLYMERS
卷 211, 期 -, 页码 349-359出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2019.01.111
关键词
Glucan; Glucose; Biofuels; Autohydrolysis; Severity factor; Biorefinery
资金
- Secretary of Public Education of Mexico - Mexican Science and Technology Council (SEP-CONACYT) [2015-01, 254808]
- National Council for Science and Technology (CONACYT, Mexico) [611312/452636]
- Portuguese Foundation for Science and Technology (FCT) [SFRH/BPD/100786/2014]
One of the major challenges in biofuels production from lignocellulosic biomass is the generation of high glucose titers from cellulose in the enzymatic hydrolysis stage of pretreated biomass to guarantee a cost-effective process. Therefore, the enzymatic saccharification on cellulose at high solid loading is an alternative. In this work, the agave bagasse was hydrothermally pretreated and optimized at 194 degrees C/30 min, obtaining a pretreated solid rich in cellulose content (> 46.46%), and subjected to enzymatic hydrolysis at high solid levels. A horizontal bioreactor was designed for enzyme saccharification at high solid loadings [25% (w/v)]. The bioreactor improved mixing efficiency, with cellulose conversions up to 98% (195.6 g/L at 72 h). Moreover, mathematical modeling of cellulase deactivation demonstrated that cellulases lose most of their initial activity in the first hours of the reaction. Also, cellulose was characterized by X-ray diffraction, and the pretreated solids were visualized using scanning electron microscopy.
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