期刊
BRAZILIAN JOURNAL OF MICROBIOLOGY
卷 50, 期 3, 页码 603-612出版社
SPRINGER
DOI: 10.1007/s42770-019-00083-5
关键词
Biocontrol; Trichoderma sp; Yeast expression; Hydrophobin; Populus davidiana x P; alba var; pyramidalis
类别
资金
- National High Technology Research and Development Program (the 13th Five-Year Plan Program) [2016YFC0501505]
- Fundamental Research Funds of the Central University, China [2572017CA06, 2572014BA15]
Herein, the class II hydrophobin gene HFBII-4 was cloned from the biocontrol agent Trichoderma asperellum ACCC30536 and recombinant rHFBII-4 was expressed in Pichia pastoris GS115. Treatment of Populus davidiana x P. alba var. pyramidalis (PdPap poplar) with rHFBII-4 altered the expression levels of genes in the auxin, salicylic acid (SA), and jasmonic acid (JA) signal transduction pathways. Polyphenol oxidase (PPO) and phenylalanine ammonia lyase (PAL) enzyme activities were induced with rHFBII-4. Evans Blue and nitro blue tetrazolium (NBT) staining indicated that cell membrane permeability and reactive oxygen species were lower in the leaves of plants treated with rHFBII-4. The chlorophyll content was higher than that of control at 2-5 days after treatment. Furthermore, poplar seedlings were inoculated with Alternaria alternata, disease symptoms were observed. The diseased area was smaller in leaves induced with rHFBII-4 compared with control. In summary, rHFBII-4 enhances resistance to A. alternata.
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