期刊
BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH
卷 52, 期 5, 页码 -出版社
ASSOC BRAS DIVULG CIENTIFICA
DOI: 10.1590/1414-431X20198108
关键词
Animal models; Gene editing; Atherosclerosis
资金
- Russian Science Foundation [17-75-20249]
- Russian Science Foundation [17-75-20249] Funding Source: Russian Science Foundation
Animal models of diseases are invaluable tools of modern medicine. More than forty years have passed since the first successful experiments and the spectrum of available models, as well as the list of methods for creating them, have expanded dramatically. The major step forward in creating specific disease models was the development of gene editing techniques, which allowed for targeted modification of the animal's genome. In this review, we discuss the available tools for creating transgenic animal models, such as transgenesis methods, recombinases, and nucleases, including zinc finger nuclease (ZFN), transcription activator-like effector nuclease (TALEN), and CRISPR/Cas9 systems. We then focus specifically on the models of atherosclerosis, especially mouse models that greatly contributed to improving our understanding of the disease pathogenesis and we outline their characteristics and limitations.
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