Artesunate promotes Th1 differentiation from CD4+ T cells to enhance cell apoptosis in ovarian cancer via miR-142

The aim of this study was to evaluate the influence of artesunate on Th1 differentiation and its anti-tumor effect on ovarian cancer. A Murine ovarian cancer model was established by ID8 cells transplantation. The expression of miR-142 and Sirt1 proteins in peripheral CD4(+) T cells were quantified with qRT-PCR and western blot, respectively. Peripheral CD4(+) T cells were induced for Th1 differentiation. The percentages of apoptosis of Th1/CD4(+) T cells and ovarian cancer cells were analyzed by flow cytometry. The IFN-gamma level was examined through enzyme-linked immunosorbent assay. Artesunate promoted miR-142 expression in peripheral CD4(+) T cells and Th1 differentiation from CD4(+) T cells. Artesunate promoted cell apoptosis of ovarian cancer cells by inducing Th1 differentiation. By up-regulating miR-142, artesunate suppressed Sirt1 level and promoted Th1 differentiation. Artesunate enhanced the pro-apoptotic effects of Th1 cells on ovarian cancer via the miR-142/Sirt1 pathway. Artesunate promoted Th1 differentiation from CD4(+) Tcells by down-regulating Sirt1 through miR-142, thereby enhancing cell apoptosis in ovarian cancer.