期刊
BIOTECHNOLOGY FOR BIOFUELS
卷 12, 期 -, 页码 -出版社
BMC
DOI: 10.1186/s13068-019-1458-z
关键词
Microalgae; Phosphorus; Hormesis; Nitrogen limitation; Toxic
资金
- National Natural Science Foundation of China [51708095, 51578117, 51722803]
- Fundamental Research Funds for the Central Universities [2412017QD027, 2412018ZD013, 2412018ZD042]
- Science and Technology Project of Jilin Province [20180520168JH]
- China Postdoctoral Science Foundation [2017M611302, 2018T110241]
BackgroundPhosphorus (P) is an essential element of microalgae, which is either required for anabolism or for energy metabolism. When employing a nitrogen limitation strategy to trigger microalgal intracellular lipid accumulation, P supplementation was always simultaneously applied to compensate for the accompanied growth inhibition.ResultsThis study identified that P exerts hormesis effects on microalgae. Slight excess of P (45mgL(-1)) under nitrogen limitation condition stimulated the cell growth of Chlorella regularis and achieved a 10.2% biomass production increase. This also improved mitochondrial activity by 25.0% compared to control (P=5.4mgL(-1)). The lipid productivity reached 354.38mg(Ld)(-1), which increased by 39.3% compared to control. Such an improvement was caused by the intracellularly stored polyphosphate energy pool. However, large excess of P (250mgL(-1)) inhibited the cell growth by 38.8% and mitochondrial activity decreased by 71.3%. C. regularis cells showed obvious poisoning status, such as enlarged size, plasmolysis, deformation of cell walls, and disorganization of organelles. This is probably because the over-accumulated P protonated the amide-N and disrupted membrane permeability.ConclusionsThese results provide new insight into the roles of P in microalgae lipid production: P does not always play a positive role under nitrogen limitation conditions.
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