期刊
BIOTECHNOLOGY AND BIOENGINEERING
卷 116, 期 8, 页码 1901-1908出版社
WILEY
DOI: 10.1002/bit.26993
关键词
biofuels; bioreactor; Caldicellulosiruptor bescii; lignocellulose; switchgrass
资金
- National Science Foundation [CBET-1264053, CBET-1264052]
- U.S. Department of Energy
- US Department of Education GAANN Fellowship [P200A160061]
The extreme thermophile Caldicellulosiruptor bescii solubilizes and metabolizes the carbohydrate content of lignocellulose, a process that ultimately ceases because of biomass recalcitrance, accumulation of fermentation products, inhibition by lignin moieties, and reduction of metabolic activity. Deconstruction of low loadings of lignocellulose (5g/L), either natural or transgenic, whether unpretreated or subjected to hydrothermal processing, by C. bescii typically results in less than 40% carbohydrate solubilization. Mild alkali pretreatment (up to 0.09g NaOH/g biomass) improved switchgrass carbohydrate solubilization by C. bescii to over 70% compared to less than 30% for no pretreatment, with two-thirds of the carbohydrate content in the treated switchgrass converted to acetate and lactate. C. bescii grown on high loadings of unpretreated switchgrass (50g/L) retained in a pH-controlled bioreactor slowly purged (tau=80hr) with growth media without a carbon source improved carbohydrate solubilization to over 40% compared to batch culture at 29%. But more significant was the doubling of solubilized carbohydrate conversion to fermentation products, which increased from 40% in batch to over 80% in the purged system, an improvement attributed to maintaining the bioreactor culture in a metabolically active state. This strategy should be considered for optimizing solubilization and conversion of lignocellulose by C. bescii and other lignocellulolytic microorganisms.
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