期刊
BIOSENSORS & BIOELECTRONICS
卷 132, 期 -, 页码 238-247出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2019.02.062
关键词
Smart Hg2+ detection method; Thymine-Hg2+-thymine coordination chemistry; Smartphone fluorescence microscope; Quantitative Hg2+ measurements; On-site detection
类别
资金
- National Natural Science Foundation of China [31870154, 31522056, 61705092]
- National Key Research and Development Program [2015BAD12B01, 2018YFD0500100]
- Natural Science Foundation of Jiangsu Province of China [BK20170194]
- Shanghai Sailing Program [17YF1407000]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
Mercury is a bio-accumulative and toxic pollutant causing severe damages to human health and environment. Since Hg2+ is the most stable form of mercury, selective and sensitive Hg2+ detection is required. Though classical approaches can realize accurate Hg2+ detection, the complicated instruments and the time-consuming operations inevitably limit their on-site applications. Here, we design a smart Hg2+ detection approach using the fluorescence biosensor, the smartphone fluorescence microscope and the smartphone application for Hg2+ on site detection. Based on the thymine-Hg2+-thyrnine coordination chemistry, a selective and sensitive fluorescence biosensor is designed for capturing Hg2+ in aqueous solution; besides, a miniaturized smartphone fluorescence microscope for fluorescence signal collection and an image processing application for quantitative Hg2+ measurements are constructed. A highly specific detection of Hg2+ with a linear relation between 1 nM and 1 M with a limit of detection of 1 nM is obtained using the smart Hg2+ detection approach. Considering it can realize selective and sensitive quantitative Hg2+ measurements in high precision with simple operations and cost-effective system, it is believed the proposed smart Hg2+ detection approach owns great potentials in Hg2+ detection for routine uses at home and in the field.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据