期刊
BIOPROCESS AND BIOSYSTEMS ENGINEERING
卷 42, 期 8, 页码 1367-1374出版社
SPRINGER
DOI: 10.1007/s00449-019-02136-3
关键词
Adaptive evolution; Enzymatic saccharification; Ethanol production; Fermentation; Hyper-thermal acid hydrolysis; Water hyacinth (Eichhornia crassipes)
资金
- Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Education [2016R1D1A1A09918683]
- National Research Foundation of Korea [2016R1D1A1A09918683] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Water hyacinth (Eichhornia crassipes) was used as a feedstock for ethanol production. The optimal hyper-thermal (HT) acid hydrolysis conditions were 8% (w/v) slurry content, 200mM H2SO4, at 160 degrees C for 20min and enzymatic saccharification for 48h using an enzyme mixture of 20units/mL Viscozyme L and Cellic C Tec2. After pretreatment, 48.2g/L monosaccharides were obtained. Fermentation was conducted with wild and adapted Saccharomyces cerevisiae, Pichia stipitis and Candida lusitaniae. Wild-type S. cerevisiae, P. stipitis, and C. lusitaniae produced 15.3, 19.5 and 22.7g/L of ethanol, respectively. Adaptive evolution was carried out on 6% (w/v) xylose. S. cerevisiae, P. sipitis and C. lusitaniae adapted to xylose produced 15.3, 21.4 and 23.9g/L of ethanol with Y-EtOH of 0.32, 0.44 and 0.49, respectively. These results indicate that water hyacinth has potential as a feed stock for ethanol.
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