期刊
BIOLOGY OF REPRODUCTION
卷 101, 期 3, 页码 617-634出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/biolre/ioz088
关键词
single-cell; transcriptome; stem cells; heterogeneity; testis
资金
- NICHD NIH HHS [R01 HD090007] Funding Source: Medline
Mammalian spermatogenesis is a complex developmental program that transforms mitotic testicular germ cells (spermatogonia) into mature male gametes (sperm) for production of offspring. For decades, it has been known that this several-weeks-long process involves a series of highly ordered and morphologically recognizable cellular changes as spermatogonia proliferate, spermatocytes undertake meiosis, and spermatids develop condensed nuclei, acrosomes, and flagella. Yet, much of the underlying molecular logic driving these processes has remained opaque because conventional characterization strategies often aggregated groups of cells to meet technical requirements or due to limited capability for cell selection. Recently, a cornucopia of single-cell transcriptome studies has begun to lift the veil on the full compendium of gene expression phenotypes and changes underlying spermatogenic development. These datasets have revealed the previously obscured molecular heterogeneity among and between varied spermatogenic cell types and are reinvigorating investigation of testicular biology. This review describes the extent of available single-cell RNA-seq profiles of spermatogenic and testicular somatic cells, how those data were produced and evaluated, their present value for advancing knowledge of spermatogenesis, and their potential future utility at both the benchtop and bedside. Summary Sentence This review details the host of new and revolutionary single-cell RNA-seq results from mouse and human spermatogenic cells that are already informing basic biological concepts of testicular function with high translational significance for male infertility and contraception.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据