期刊
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
卷 1867, 期 10, 页码 980-987出版社
ELSEVIER
DOI: 10.1016/j.bbapap.2019.04.007
关键词
Intrinsically disordered proteins; Liquid-liquid phase separation; Single-molecule biophysics; Single-molecule FRET
资金
- US NIH [R35 GM30375, RO1 GM066833, RO1 GM115634, RO1 CA214054]
- US National Science Foundation [1818385]
- Swedish Research Council (Vetenskapsrklet) International Postdoctoral Fellowship [2016-06672]
- Direct For Biological Sciences
- Div Of Molecular and Cellular Bioscience [1818385] Funding Source: National Science Foundation
- Swedish Research Council [2016-06672] Funding Source: Swedish Research Council
Intrinsically disordered proteins (IDPs) are ubiquitous in proteomes and serve in a range of cellular functions including signaling, regulation, transport and enzyme function. IDP misfunction and aggregation are also associated with several diseases including neurodegenerative diseases and cancer. During the past decade, single molecule methods have become popular for detailed biophysical and structural studies of these complex proteins. This work has included recent applications to cellular liquid-liquid phase separation (LLPS), relevant for functional dynamics of membraneless organelles such as the nucleolus and stress granules. In this concise review, we cover the conceptual motivations for development and application of single-molecule fluorescence methods for such IDP studies. We follow with a few key examples of systems and biophysical problems that have been addressed, and conclude with thoughts for emerging and future directions.
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