期刊
ARQUIVO BRASILEIRO DE MEDICINA VETERINARIA E ZOOTECNIA
卷 71, 期 1, 页码 11-20出版社
ARQUIVO BRASILEIRO MEDICINA VETERINARIA ZOOTECNIA
DOI: 10.1590/1678-4162-10216
关键词
pigs; explants; intestine; probiotic
资金
- CNPq
- Capes/Fundacao Araucaria fellowship
In the present study, histological, morphometrical and ultrastructural analysis were performed to investigate intestinal mucosa changes in piglets jejunal explants exposed to two concentration of heat-inactivated Lactobacillus plantarum and their respective culture supernatants. Jejunal explants were incubated for 4 hours in DMEM culture medium with a) only culture medium (control group), b) heat-inactivated Lactobacillus plantarum strain1 - LP1 (1.1 x 10(8)CFU/ml), c) heat-inactivated Lactobacillus plantarum strain2 - LP2 (2.0 x 10(9)CFU/ml), d) heat-inactivated Lactobacillus plantarum strain1 culture supernatant (CS1), and e) heat-inactivated Lactobacillus plantarum strain2 culture supernatant (CS2). Explants exposed to heat-inactivated L. plantarum strain 1 and 2 showed multifocal to difuse villi atrophy, villi apical necrosis and enterocyte flattening. Morphological assessment revealed similar results with bacterial adhesion to mucus and intestinal epithelial cells and, morphometric analysis showed a decreased villi height compared to the control group. Alterations in explants treated with the culture supernatant of both strains include mild villi atrophy and mild enterocyte apical necrosis. Morphological assesment reveled numerous well delineated villi and, morphometric analysis showed a significant increase in villi height compared to the control group. In general, exposure to the culture supernatants improved the intestinal morphology.
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