期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 58, 期 23, 页码 7606-7610出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201901412
关键词
antibodies; cancer; circulating tumor cells; microfluidics; simulation
资金
- McJunkin Family Charitable Foundation
- University of Florida (UF) Division of Sponsored Research
- UF Health Cancer Center
- National Cancer Institute [K25CA149080]
- NIH TL1 Grant under the UF Clinical and Translational Science Awards
Circulating tumor cells (CTCs) are an important biomarker for cancer prognosis and treatment monitoring. However, the heterogeneity of the physical and biological properties of CTCs limits the efficiency of various approaches used to isolate small numbers of CTCs from billions of normal blood cells. To address this challenge, we developed a lateral filter array microfluidic (LFAM) device to integrate size-based separation with immunoaffinity-based CTC isolation. The LFAM device consists of a serpentine main channel, through which most of a sample passes, and an array of lateral filters for CTC isolation. The unique device design produces a two-dimensional flow, which reduces nonspecific, geometric capture of normal cells as typically observed in vertical filters. The LFAM device was further functionalized by immobilizing antibodies that are specific to the target cells. The resulting devices captured pancreatic cancer cells spiked in blood samples with (98.7 +/- 1.2)% efficiency and were used to isolate CTCs from patients with metastatic colorectal cancer.
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