期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 58, 期 24, 页码 7972-7976出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201903058
关键词
biological activity; fluorescent probes; imaging agents; microglia; structure-activity relationships
资金
- A*STAR (Agency for Science, Technology and Research, Singapore) Biomedical Research Council
- Joint Council Office Development Program, A*STAR [1334k00083]
- Institute for Basic Science (IBS) [IBS-R007-A1]
- Singapore National Research Foundation Competitive Research Program [NRF-CRP17-2017-04]
- National Medical Research Council Open-Fund Individual Research Grant [NMRC/OFIRG/0050/2017]
- Duke-NUS Signature Research Program Block Grant
- Khoo Postdoctoral Fellowship Award [Duke-NUS-KPFA/2016/0007]
- Singapore Immunology Network (SIgN) core funding
- Singapore National Research Foundation Senior Investigatorship (NRFI) [NRF2016NRF-NRFI001-02]
- BMRC [IAF 311006, H16/99/b0/011]
- [SUTD-T1SRCI17126]
Microglia, the brain-resident macrophage, are involved in brain development and contribute to the progression of neural disorders. Despite the importance of microglia, imaging of live microglia at a cellular resolution has been limited to transgenic mice. Efforts have therefore been dedicated to developing new methods for microglia detection and imaging. Using a thorough structure-activity relationships study, we developed CDr20, a high-performance fluorogenic chemical probe that enables the visualization of microglia both in vitro and in vivo. Using a genome-scale CRISPR-Cas9 knockout screen, the UDP-glucuronosyltransferase Ugt1a7c was identified as the target of CDr20. The glucuronidation of CDr20 by Ugt1a7c in microglia produces fluorescence.
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