4.8 Article

Visualizing Microglia with a Fluorescence Turn-On Ugt1a7c Substrate

期刊

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 58, 期 24, 页码 7972-7976

出版社

WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201903058

关键词

biological activity; fluorescent probes; imaging agents; microglia; structure-activity relationships

资金

  1. A*STAR (Agency for Science, Technology and Research, Singapore) Biomedical Research Council
  2. Joint Council Office Development Program, A*STAR [1334k00083]
  3. Institute for Basic Science (IBS) [IBS-R007-A1]
  4. Singapore National Research Foundation Competitive Research Program [NRF-CRP17-2017-04]
  5. National Medical Research Council Open-Fund Individual Research Grant [NMRC/OFIRG/0050/2017]
  6. Duke-NUS Signature Research Program Block Grant
  7. Khoo Postdoctoral Fellowship Award [Duke-NUS-KPFA/2016/0007]
  8. Singapore Immunology Network (SIgN) core funding
  9. Singapore National Research Foundation Senior Investigatorship (NRFI) [NRF2016NRF-NRFI001-02]
  10. BMRC [IAF 311006, H16/99/b0/011]
  11. [SUTD-T1SRCI17126]

向作者/读者索取更多资源

Microglia, the brain-resident macrophage, are involved in brain development and contribute to the progression of neural disorders. Despite the importance of microglia, imaging of live microglia at a cellular resolution has been limited to transgenic mice. Efforts have therefore been dedicated to developing new methods for microglia detection and imaging. Using a thorough structure-activity relationships study, we developed CDr20, a high-performance fluorogenic chemical probe that enables the visualization of microglia both in vitro and in vivo. Using a genome-scale CRISPR-Cas9 knockout screen, the UDP-glucuronosyltransferase Ugt1a7c was identified as the target of CDr20. The glucuronidation of CDr20 by Ugt1a7c in microglia produces fluorescence.

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