期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 58, 期 24, 页码 8092-8096出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201902960
关键词
analytical methods; droplet microfluidics; ionophores; molecular recognition; sensors
资金
- Exercise and Sport Science Initiative Pilot Grant at the University of Michigan [ESSI-2018-6]
- National Institutes of Health [CA191186]
- NSF MRI-ID award [DBI-0959823]
Droplet microfluidics is an enabling platform for high-throughput screens, single-cell studies, low-volume chemical diagnostics, and microscale material syntheses. Analytical methods for real-time and in situ detection of chemicals in the droplets will benefit these applications, but they remain limited. Reported herein is a novel heterogeneous chemical sensing strategy based on functionalization of the oil phase with rationally combined sensing reagents. Sub-nanoliter oil segments containing pH-sensitive fluorophores, ionophores, and ion-exchangers enable highly selective and rapid fluorescence detection of physiologically important electrolytes (K+, Na+, and Cl-) and polyions (protamine) in sub-nanoliter aqueous droplets. Electrolyte analysis in whole blood is demonstrated without suffering from optical interference from the sample matrix. Moreover, an oil phase doped with an aza-BODIPY dye allows indication of H2O2 in the aqueous droplets, exemplifying sensing of targets beyond ionic species.
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