期刊
ANALYTICAL CHEMISTRY
卷 91, 期 9, 页码 6296-6303出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.9b01064
关键词
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资金
- Natural Science Foundation of China [21777154, 91851101]
- National Key Research and Development Program of China [2017YFE0107300, 2017YFD0200201]
- K. C. Wong Education Foundation
Speeding up antibiotic susceptibility testing (AST) is urgently needed in clincial settings to guide fast and tailored antibiotic prescription before treatment. It remains a big challenge to achieve a sample-to-AST answer within a half working day directly from a clinical sample. Here we develop single-cell Raman spectroscopy coupled with heavy water labeling (Raman-D2O) as a rapid activity-based AST approach directly applicable for clinical urine samples. By rapidly transferring (15 min) bacteria in clinical urine for AST, the total assay time from receiving urine to binary susceptibility/resistance (S/R) readout was shortened to only 2.5 h. Moreover, by overcoming the nonsynchronous responses between microbial activity and microbial growth, together with setting a new S/R cutoff value based on relative C D ratios, S/R of both pathogenic isolates and three clinical urines against antibiotics of different action mechanisms determined by Raman-D2O were all consistent with the slow standard AST assay used in clincial settings. This work promotes clinical practicability and faciliates antibiotic stewardship.
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