期刊
ANALYTICAL CHEMISTRY
卷 91, 期 9, 页码 5542-5547出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.9b00734
关键词
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资金
- Netherlands Organization for Scientific Research (NWO) through a VIDI grant [723.013.008]
- Roadmap Initiative Proteins@Work - Netherlands Organization for Scientific Research (NWO) [184.032.201]
- MSMed program - European Union's Horizon 2020 Framework Programme [686547]
Recent technological advances have made it possible to investigate the hitherto rather elusive protein histidine phosphorylation. However, confident site-specific localization of protein histidine phosphorylation remains challenging. Here, we address this problem, presenting a mass-spectrometry-based approach that outperforms classical HCD fragmentation without compromising sensitivity. We use the phosphohistidine immonium ion as a diagnostic tool as well as ETD-based fragmentation techniques to achieve unambiguous identification and localization of histidine-phosphorylation sites. The work presented here will allow more confident investigation of the phosphohistidine proteome to reveal the roles of histidine phosphorylation in cellular signaling events.
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