期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 411, 期 19, 页码 4559-4567出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-019-01753-x
关键词
Mature; precursor microRNAs; Aptamer-targeted; Molecular beacon; MoS2 nanosheet; Intercellular imaging
资金
- Major Program of the National Natural Science Foundation of China [21890742]
- National Natural Science Foundation of China [21874008, 21475008]
- Special Foundation for State Major Research Program of China [2016YFC0106602, 2016YFC0106601]
- Open Research Fund Program of the Beijing Key Lab of Plant Resource Research and Development, Beijing Technology and Business University [PRRD-2016-YB2]
- Fundamental Research Funds for the Central Universities [FRF-BD-17-016A]
- Beijing Municipal Science and Technology Commission [z131102002813058]
Mature microRNAs (miRNAs) are small-sized RNAs cleaved from precursor microRNAs (pre-miRNAs) by the RNase Dicer. Various miRNAs play key regulatory roles in tumorigenesis and metastasis, and are therefore potential diagnostic and prognostic cancer biomarkers. However, detecting miRNAs and pre-miRNAs accurately and selectively in living cells remains a major challenge, as the mature miRNA sequence is also present in its pre-miRNA and current sequence probes exhibit poor gene delivery efficiency. Herein, we report a strategy for selectively and accurately detecting miRNA-21 and pre-miRNA-21 in living cells using functional MoS2 nanosheets (NSs) loaded with rationally engineered molecular beacons (MBs). The exfoliated MoS2 nanosheets (NSs) with a mean lateral diameter of 50-70nm were functionalized with the aptamer AS1411 and polyethylene glycol (MoS2-PEG-AS) to achieve target-cell-specific delivery and to enhance biocompatibility. The large available surface of the MoS2-PEG-AS was loaded with MB probes. The resulting MoS2-PEG-AS/MBs present cancer-cell-targeting ability, good protection properties, good optical stability, and biocompatibility. We demonstrated that the resulting nanoprobes can selectively image miRNA-21 and pre-miRNA-21 in various cell lines by facilitating enhanced fluorescence in the presence of miRNA-21 and pre-miRNA-21. Thus, these MoS2-PEG-AS/MBs are potentially a tool to discriminate between intracellular miRNA and pre-miRNA at different expression levels.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据