期刊
ANALYTICA CHIMICA ACTA
卷 1077, 期 -, 页码 232-242出版社
ELSEVIER
DOI: 10.1016/j.aca.2019.05.038
关键词
Point-of-care; Label-free multiplexed biosensing; Nanoplasmonics; Sepsis; Protein and miRNA biomarkers; Microarray
资金
- European Union's Horizon 2020 Research and Innovation program (RAIS Project) [644956]
- CERCA program / Generalitat de Catalunya
- Severo Ochoa program from Spanish MINECO [SEV-2017-0706]
- FEDER funds from the European Regional Development Funds program [FICTS-1420-27]
- SNSF [178438]
- Spanish Ministry of Economy and Competitiveness through the Severo Ochoa Programme for Centres of Excellence in RD [SEV-2015-0522]
- Spanish Ministry of Economy and Competitiveness through OPTO-SCREEN [TEC2016-75080-R]
- Fundacio Privada Cellex
- Generalitat de Catalunya through the CERCA program
- AGAUR [2017 SGR 1634]
y Sepsis is a condition characterized by a severe stage of blood-infection often leading to tissue damage, organ failure and finally death. Fast diagnosis and identification of the sepsis stage (sepsis, severe sepsis or septic shock) is critical for the patient's evolution and could help in defining the most adequate treatment in order to reduce its mortality. The combined detection of several biomarkers in a timely, specific and simultaneous way could ensure a more accurate diagnosis. We have designed a new optical point-of-care (POC) device based on a phase-sensitive interferometric biosensor with a label-free microarray configuration for potential high-throughput evaluation of specific sepsis biomarkers. The sensor chip, which relies on the use of metallic nanostructures, provides versatility in terms of biofunctionalization, allowing the efficient immobilization of different kind of receptors such as antibodies or oligonucleotides. We have focused on two structurally different types of biomarkers: proteins, including C-reactive protein (CRP) and Interleukin 6 (IL6), and miRNAs, using miRNA-16 as an example. Limits of Detection (LoD) of 18 mu g mL(-1), 88 mu g mL(-1) and 1 mM (6 mu g mL(-1)) have been respectively obtained for CRP, IL6 and miRNA-16 in individual assays, with high accuracy and reproducibility. The multiplexing capabilities have also been assessed with the simultaneous analysis of both protein biomarkers. (C) 2019 Elsevier B.V. All rights reserved.
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