期刊
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
卷 61, 期 5, 页码 575-583出版社
AMER THORACIC SOC
DOI: 10.1165/rcmb.2018-0351OC
关键词
chronic bronchitis; emphysema; pulmonary progenitor cells; lung spheroids; immunomodulation
资金
- Instituto de Salud Carlos III [PI15/00799, CP16/00039]
- Recercaixa-2012 [AA084096]
- La Sociedad Espanola de Neumologia y Cirugia Toracica [PI068/2015, PI192/2012]
- AstraZeneca Foundation COPD Young Researchers Award
- Menarini
- Centres de Recerca de Catalunya Program/Generalitat de Catalunya (FI-DGR 2016)
Tobacco smoking is the main environmental risk factor for chronic obstructive pulmonary disease (COPD), but not all smokers develop the disease. A population of lung-resident mesenchymal stem cells (LR-MSCs) exist in healthy lungs, but how tobacco smoking affects them and their role in COPD have not been assessed yet. Using a sphere-based culture technique, we isolated LR-MSCs from lung tissue obtained from nonsmokers and current and former smokers with and without COPD (n = 53). The cells were characterized by flow cytometry and Affymetrix arrays. Their immunomodulatory capacity was assessed in vitro using cocultures with T cells and after preincubation with 2.5% and 5% cigarette smoke extract. We were able to isolate LR-MSCs expressing similar phenotypic markers in all of the study groups. LR-MSCs from current smokers with COPD expressed different levels of CX3CL1 and CCL5 cytokines, and were unable to modulate CD8(+) T-cell proliferation. Preincubation of LR-MSCs with cigarette smoke extract reduced their immunomodulatory capacity. In conclusion, 1) LR-MSCs can be isolated in similar amounts from never-smokers and smokers with and without COPD; 2) their immunomodulatory capacity is impaired in current smokers with COPD, but not in those with normal lung function; and 3) this is reversible after smoking cessation and is reproducible in vitro.
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