4.6 Article

DNA hydroxymethylation combined with carotid plaques as a novel biomarker for coronary atherosclerosis

期刊

AGING-US
卷 11, 期 10, 页码 3170-3181

出版社

IMPACT JOURNALS LLC
DOI: 10.18632/aging.101972

关键词

methylation; hydroxymethylation; carotid plaque; coronary atherosclerosis

资金

  1. National Natural Science Foundation of China [81570212, 31800976]
  2. Basic Research and Frontier Exploration Project of Chongqing [cstc2018jcyjAX0058]
  3. Science and Technology Plan Project of Chongqing Yuzhong District [20140111]
  4. Chinese Medicine Science and Technology Project of Chongqing Health and Family Planning Commission [ZY201702073]
  5. Medical Research Project of Chongqing Health Bureau [2012-1-012]
  6. First Affiliated Hospital of Chongqing Medical University Cultivation Fund [PYJJ2017-28]
  7. China Cardiovascular AssociationCardiac Rehabilitation and Metabolic Therapy Research Fund [CCA-CRMT-1610]
  8. ACCESS Fund of China Heart House - China Cardiovascular Association [2018-CCA-AF-034, ky-2017-07-08]
  9. Medical Research Project of Chongqing Health and Family Planning Commission [2015ZDXM003]
  10. Chongqing Science and Health Joint Medical Research Project [2018QNXM024]

向作者/读者索取更多资源

Little is known about the diagnostic value of DNA methylation and hydroxymethylation for coronary atherosclerosis. Carotid plaque is a common marker for coronary atherosclerosis. Our aim is to determine whether DNA methylation and hydroxymethylation combined with carotid plaques can be useful to the diagnosis of coronary atherosclerosis. The 5-methylcytosine (5-mC) and 5-hydroxymethylcytosine (5-hmC) levels from peripheral blood mononuclear cells (PBMCs) were measured in 113 enrolled patients. Crouse score and Gensini score were used to evaluate the severity of carotid and coronary atherosclerosis, respectively. With the increasing of severity of carotid plaque, a stepwise upward trend was observed in 5-mC and 5-hmC levels from PBMCs, which were significantly correlated with the risk factors, Crouse score and Gensini score. Crouse score and 5-hmC, not 5-mC, were the risk factors for coronary atherosclerosis after adjustment for the risk factors (the history of diabetes, FPG and HbA1c). Receiver operating characteristic (ROC) analysis indicated that 5-hmC combined with Crouse score was the diagnostic biomarker for coronary atherosclerosis, with the highest areas under the curve (AUC) for 0.980 (0.933-0.997), valuable sensitivity for 96.23% and specificity for 91.67%. These findings suggest 5-hmC level combined with Crouse score may provide the meaningful information for coronary atherosclerosis diagnosis.

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