期刊
ADVANCES IN POLYMER TECHNOLOGY
卷 -, 期 -, 页码 -出版社
WILEY-HINDAWI
DOI: 10.1155/2019/7632873
关键词
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资金
- National Natural Science Foundation, China [81473172, 81503025, 81773695, 81720108030, 81273470]
- Doctoral Fund of Ministry of Education of China [20123227110009]
- Special Funds for 333 projects [BRA2013198]
- Industry-University-Research Institution Cooperation in Jiangsu Province [JHB2012-37, GY2012049, GY2013055]
- Industry-University-Research Institution Cooperation in Zhenjiang City [JHB2012-37, GY2012049, GY2013055]
- senior professionals' foundation of Jiangsu University projects [13JDG007]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
- Jiangsu Province Postdoctoral Foundation [1701068C]
- China Postdoctoral Foundation [2017M621659]
An innovative strategy for the generation of chondrocytes was thoroughly studied in this paper. Polyetherimide-modified polysaccharides of Porphyra yezoensis (pmPPY) served as a nonviral gene vector and delivered Sox9 plasmid to directly reprogram mouse embryonic fibroblasts into chondrocytes. The gene transfer efficiency was evaluated through ELISA, RT-PCR, and Western blot. The induced chondrocytes were identified through toluidine blue, Safranin O, and the immunostaining. The expression level of collagen II was finally evaluated through western blot. The pSox9/pmPPY nanoparticles (1:50) showed lower cytotoxicity as well as greater gene transfection efficiency than Lipofectamine 2000 and polyetherimide (PEI) (p<0.05). The results of toluidine blue, Safranin O, and the immunostaining of collagen II further showed that the normal MEFs were successfully reprogrammed into chondrocytes. These findings indicate that pmPPY could be a promising gene vector for the generation of chondrocytes via single-gene delivery strategy, which might provide abundant chondrocytes for cartilage repair.
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