期刊
ADVANCED MATERIALS
卷 31, 期 25, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/adma.201808355
关键词
3D biological imaging; angiography; conjugated polymer nanoparticles; microfluidics; photoacoustic imaging
类别
资金
- Singapore NRF Competitive Research Program [R279-000-483-281]
- National Natural Science Foundation of China (NSFC) [81801758, 81771841, 91739117, 81522024, 81427804, 81430038, 61475182]
- National University of Singapore [R279-000-482-133]
- NRF [R279-000-444-281]
Exogenous contrast-agent-assisted NIR-II optical-resolution photoacoustic microscopy imaging (ORPAMI) holds promise to decipher wide-field 3D biological structures with deep penetration, large signal-to-background ratio (SBR), and high maximum imaging depth to depth resolution ratio. Herein, NIR-II conjugated polymer nanoparticle (CP NP) assisted ORPAMI is reported for pinpointing cerebral and tumor vasculatures. The CP NPs exhibit a large extinction coefficient of 48.1 L g(-1) at the absorption maximum of 1161 nm, with an ultrahigh PA sensitivity up to 2 mu g mL(-1). 3D ORPAMI of wide-field mice ear allows clear visualization of regular vasculatures with a resolution of 19.2 mu m and an SBR of 29.3 dB at the maximal imaging depth of 539 mu m. The margin of ear tumor composed of torsional dense vessels among surrounding normal regular vessels can be clearly delineated via 3D angiography. In addition, 3D whole-cortex cerebral vasculatures with large imaging area (48 mm(2)), good resolution (25.4 mu m), and high SBR (22.3 dB) at a depth up to 1001 mu m are clearly resolved through the intact skull. These results are superior to the recently reported 3D NIR-II fluorescence confocal vascular imaging, which opens up new opportunities for NIR-II CP-NP-assisted ORPAMI in various biomedical applications.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据