期刊
ACS NANO
卷 13, 期 5, 页码 5015-5027出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsnano.9b01025
关键词
nanostructures; DNA origami; DNA nanotechnology; self-assembly; phagemid
类别
资金
- European Research Council [724261]
- Deutsche Forschungsgemeinschaft
- [SFB863 TPA9]
- European Research Council (ERC) [724261] Funding Source: European Research Council (ERC)
DNA origami nano-objects are usually designed around generic single-stranded scaffolds. Many properties of the target object are determined by details of those generic scaffold sequences. Here, we enable designers to fully specify the target structure not only in terms of desired 3D shape but also in terms of the sequences used. To this end, we built design tools to construct scaffold sequences de novo based on strand diagrams, and we developed scalable production methods for creating design-specific scaffold strands with fully user-defined sequences. We used 17 custom scaffolds having different lengths and sequence properties to study the influence of sequence redundancy and sequence composition on multilayer DNA origami assembly and to realize efficient one-pot assembly of multiscaffold DNA origami objects. Furthermore, as examples for functionalized scaffolds, we created a scaffold that enables direct, covalent cross-linking of DNA origami via UV irradiation, and we built DNAzyme-containing scaffolds that allow postfolding DNA origami domain separation.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据