Cell-Penetrating Peptide-Functionalized Persistent Luminescence Nanoparticles for Tracking J774A.1 Macrophages Homing to Inflamed Tissues

Recruitment of leukocytes exhibiting arguable and ambiguous processes is involved in the inflammatory response, a specific reaction of organisms to tissue damage. Tracking leukocytes is of great importance for understanding the recruitment of leukocytes. Here, we report the fabrication of carboxyl silane and TAT cell-penetrating peptide-functionalized near-infrared-emitting persistent luminescence nano particles (PLNP-TAT) with deep tissue penetration in bioimaging for autofluorescence-free tracking of J774A.1 macrophages homing to inflamed tissues. The PLNP-TAT enables effective labeling of the J774A.1 macrophages and the tracking of the migration of cells to the simulated endothelial inflammatory microenvironment in vitro. Moreover, the PLNP-TAT also allows the tracking of J774A.1 macrophages homing to inflamed tissues in vivo under discontinuous illumination with a red light-emitting diode light. The PLNP-TAT allows in vivo tracking of leukocytes without the need for conventional continuous excitation and offers great potential in cell-tracking and diagnostic applications without the autofluorescence background and thermal damages brought about by continuous excitation.