期刊
ACS APPLIED MATERIALS & INTERFACES
卷 11, 期 19, 页码 17167-17176出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.9b01066
关键词
neurite; Schwann cell; decellularized nerve matrix; electrospun nanofiber; remyelination; fasciculation; topographical guidance; cell migration
资金
- National Key R&D Program of China [2016YFC1100103, 2016YFC1101603]
- National Natural Science Foundation of China [51673220, 5107378, U1134007]
- Science and Technology Planning Project of Guangdong Province, China [2015B010125001, 2015B020233012, 2017A050501017]
- Major Project of Health and Medical Collaborative Innovation of Guangzhou [201508020251]
- foreign cooperation project of Guangzhou [201807010082]
- Guangdong Innovative and Entrepreneurial Research Team Program [2013S086]
Synergistic intercellular interactions have been widely acknowledged in tuning functional cell behaviors in vivo, and these interactions have inspired the development of a variety of scaffolds for regenerative medicine. In this paper, the promotion of Schwann cell (SC)-neurite interactions through the use of a nerve extracellular matrix-coated nanofiber composite in vitro was demonstrated using a cell culturing platform consisting of either random or aligned electrospun poly(L-lactic acid) nanofibers and decellularized peripheral nerve matrix gel (pDNM gel) from porcine peripheral nervous tissue. The pDNM-coated nanofiber platform served as a superior substrate for dorsal root ganglion culturing. Furthermore, SC migration was facilitated by pDNM gel coating on the nanofibers, accompanied with much faster axonal extension, in comparison with the effect of topographical guidance from the aligned electrospun fibers only. Finally, the decellularized nerve matrix promoted the ability of SCs to wrap around bundled neurites, triggering axonal remyelination toward nerve fiber functionalization.
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