期刊
JOURNAL OF TISSUE ENGINEERING
卷 10, 期 -, 页码 -出版社
SAGE PUBLICATIONS INC
DOI: 10.1177/2041731419830264
关键词
Tissue engineering; mathematical modelling; phosphate glass; cell culture; microcarriers; bioprocessing; titanium; cobalt; biomaterials
资金
- Rosetrees Trust [A696]
- European Union [739572]
- Engineering and Physical Sciences Research Council (EPSRC) [EP/G034656/1]
Tissue engineering has the potential to augment bone grafting. Employing microcarriers as cell-expansion vehicles is a promising bottom-up bone tissue engineering strategy. Here we propose a collaborative approach between experimental work and mathematical modelling to develop protocols for growing microcarrier-based engineered constructs of clinically relevant size. Experiments in 96-well plates characterise cell growth with the model human cell line MG-63 using four phosphate glass microcarrier materials. Three of the materials are doped with 5 mol% TiO2 and contain 0%, 2% or 5% CoO, and the fourth material is doped only with 7% TiO2 (0% CoO). A mathematical model of cell growth is parameterised by finding material-specific growth coefficients through data-fitting against these experiments. The parameterised mathematical model offers more insight into the material performance by comparing culture outcome against clinically relevant criteria: maximising final cell number starting with the lowest cell number in the shortest time frame. Based on this analysis, material 7% TiO2 is identified as the most promising.
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