期刊
NATURE MICROBIOLOGY
卷 4, 期 4, 页码 587-594出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/s41564-018-0345-x
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资金
- National Institutes of Health [R01 AI083365, R01 AI099144, R01 GM076710, CETR U19 AI109764, F32 GM123579]
- Funai Overseas Scholarship
- Charles A. King Trust Postdoctoral Research Fellowship Program
The peptidoglycan cell wall is essential for the survival and morphogenesis of bacteria'. For decades, it was thought that only class A penicillin-binding proteins (PBPs) and related enzymes effected peptidoglycan synthesis. Recently, it was shown that RodA-a member of the unrelated SEDS protein family-also acts as a peptidoglycan polymerase(2-4). Not all bacteria require RodA for growth; however, its homologue, FtsW, is a core member of the divisome complex that appears to be universally essential for septa! cell wall assembly(5,6). FtsW was previously proposed to translocate the peptidoglycan precursor lipid II across the cytoplasmic membranes(7,8). Here, we report that purified FtsW polymerizes lipid II into peptidoglycan, but show that its polymerase activity requires complex formation with its partner class B PBP. We further demonstrate that the polymerase activity of FtsW is required for its function in vivo. Thus, our findings establish FtsW as a peptidoglycan polymerase that works with its cognate class B PBP to produce septal peptidoglycan during cell division.
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