期刊
NATURE MICROBIOLOGY
卷 4, 期 4, 页码 701-713出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/s41564-019-0367-z
关键词
-
类别
资金
- Danish Medical Research Council [12-124330]
- Novo Nordisk Foundation [NNF18OC0030274]
- Lundbeck Foundation [R198-2015-171]
- European Research Council [786602]
- EU FP7 MOBILEX programme [DFF - 5053-00011]
- BMBF [FKZ 01Kl1702]
- DFG [SFB/TR-84 TP C01]
- Austrian Science Fund [P 25186-B22]
- European Research Council (ERC) [786602] Funding Source: European Research Council (ERC)
The innate immune system is crucial for eventual control of infections, but may also contribute to pathology. Listeria monocytogenes is an intracellular Gram-positive bacteria and a major cause of food-borne disease. However, important knowledge on the interactions between L. monocytogenes and the immune system is still missing. Here, we report that Listeria DNA is sorted into extracellular vesicles (EVs) in infected cells and delivered to bystander cells to stimulate the cyclic guanosine monophosphate-adenosine monophosphate synthase (cGAS)-stimulator of interferon genes (STING) pathway. This was also observed during infections with Francisella tularensis and Legionella pneumophila. We identify the multivesicular body protein MVB12b as a target for TANK-binding kinase 1 phosphorylation, which is essential for the sorting of DNA into EVs and stimulation of bystander cells. EVs from Listeria-infected cells inhibited T-cell proliferation, and primed T cells for apoptosis. Collectively, we describe a pathway for EV-mediated delivery of foreign DNA to bystander cells, and suggest that intracellular bacteria exploit this pathway to impair antibacterial defence.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据