期刊
ACS SENSORS
卷 4, 期 4, 页码 938-943出版社
AMER CHEMICAL SOC
DOI: 10.1021/acssensors.9b00039
关键词
prostate cancer; pump free microfluidics; SERS; immunoassay; prostate-specific antigen
资金
- National Natural Science Foundation of China [61601165]
- China Postdoctoral Science Foundation [2018T110613]
- Fundamental Research Funds for the Central Universities [JZ2016HGBH1052]
- Anhui Key Project of Research and Development Plan [1704d0802188]
- Open Project of Faculty of Chemistry of Qingdao University of Science and Technology [QUSTHX201805]
Highly sensitive analysis of cancer biomarkers demonstrates an important impact in early diagnosis and therapies of cancer. A novel surface-enhanced Raman scattering (SERS) based immunoassay using microfluidic technique was reported for rapid analysis of prostate-specific antigen (PSA) biomarker. It is a useful screening test to discriminate prostate cancer and other diseases related to prostate. A sandwich immunoassay based on SERS nanotags, PSA biomarkers, and magnetic beads was applied on a pump-free microfluidic sensor. Magnetic immunocomplexes are isolated and trapped at the detection chamber by a permanent magnet integrated into the chip. The PBS buffer washed magnetic immunocomplexes and brought the free gold nanoparticles to the downsteam channel for waste. Our results show a good linear response in the range from 0.01 to 100 ng mL(-1). The limit of detection of the PSA level is estimated to be below 0.01 ng mL(-1) using this chip. This detection level of PSA biomarker in human serum can be accomplished in 5 min without manual incubation and a heavy syringe pump. To the best of our knowledge, this is the first SERS-based immunoassay which applied a pump-free microfluidic chip as a detection platform. We believe that the proposed method reveals a valuable potential tool for the diagnosis of prostate cancer.
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