期刊
ACS SENSORS
卷 4, 期 2, 页码 504-512出版社
AMER CHEMICAL SOC
DOI: 10.1021/acssensors.8b01607
关键词
electrochemiluminescence immunosensor; carbohydrate antigen 15-3; CQDs; graphene oxide; serum
资金
- National Natural Science Foundation of China [21765004, 21365006]
- Guangxi Science Foundation of China [2014GXNSFDA118004, 1598025-4]
- Innovation Project of Guangxi Graduate Education [YCSZ2013039]
- State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources (Guangxi Normal University) [CMEMR2017-A5]
A sensitive sandwich electrochemiluminescence immunosensor was established by employing graphene oxide-PEI-carbon quantum dots (CQDs)-Au nanohybrid as probe to measure carbohydrate antigen 15-3 (CA15-3), a breast cancer biomarker. In this work, nanocomposites of Ag nanoparticles and polydopamine (AgNPs-PDA) were synthesized by redox reaction between dopamine and Ag+. The nanocomposite with high surface area can provide an efficient substrate for immobilizing initial antibody (Ab1). Carbon quantum dots (CQDs) are fixed on polyethylenimine-functionalized graphene oxide (PEI-GO) by amide bonds. Au nanoparticles are modified on CQDs-decorated PEI-GO substrates. The secondary antibody (Ab2) was immobilized by AuNPs/CQDs-PEI-GO composite. CQDs can be assembled onto the surface of an electrode by incorporation of CA15-3 with Abl and Ab2. Under the synergistic action of AgNPs, polydopamine, AuNPs, and PEI-GO, the ECL signal of CQDs is greatly amplified as an excellent conductive material to facilitate electron transfer rate and further increase electrochemical detection capability. Under optimal conditions, the fabricated immunosensor showed a linear concentration range from 0.005 to 500 U mL(-1), with a detection limit of 0.0017 U mL(-1) (signal-to-noise ratio of 3) for CA15-3. The designed ECL immunosensor displayed receivable accuracy, excellent stability, and high specificity. The results of the detection of human serum samples are satisfactory, revealing that the method offers a potential application for the clinical diagnosis of tumor markers.
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