期刊
ACS INFECTIOUS DISEASES
卷 5, 期 6, 页码 962-973出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsinfecdis.9b00038
关键词
virus; picornavirus; enterovirus; PI4KB; OSBP; resistance
资金
- JSPS KAKENHI [16K08822]
- Advanced Research & Development Programs for Medical Innovation (AMED-CREST) Grant from the Japan Agency for Medical Research and Development, AMED [18gm0910005j0104]
- Grants-in-Aid for Scientific Research [16K08822] Funding Source: KAKEN
Phosphatidylinositol-4 kinase III beta (PI4KB) and oxysterol-binding protein (OSBP) family I provide a conserved host pathway required for enterovirus replication. Here, we analyze the role and essentiality of this pathway in enterovirus replication. Phosphatidylinositol 4-phosphate (PI4P) production and cholesterol accumulation in the replication organelle (RO) are severely suppressed in cells infected with a poliovirus (PV) mutant isolated from a PI4KB-knockout cell line (RD-[Delta PI4KB]). Major determinants of the mutant for infectivity in RD(Delta PI4KB) cells map to the A5270U(3A-R54W) and U3881C(2B-F17L) mutations. The 3A mutation is required for PI4KB-independent development of RO. The 2B mutation rather sensitizes PV to PI4KB/OSBP inhibitors by itself but confers substantially complete resistance to the inhibitors with the 3A mutation. The 2B mutation also confers hypersensitivity to interferon alpha treatment on PV. These suggest that the PI4KB/OSBP pathway is not necessarily essential for enterovirus replication in vitro. This work supports a two-step resistance model of enterovirus to PI4KB/OSBP inhibitors involving unique recessive epistasis of 3A and 2B and offers insights into a potential evolutionary pathway of enterovirus toward independence from the PI4KB/OSBP pathway.
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