Hybrid Metasurface-Based Mid-Infrared Biosensor for Simultaneous Quantification and Identification of Monolayer Protein

Metasurfaces emerge as a promising photonic platform for biosensing because they offer strong optical confinement and tunable optical resonances. Here, we show that metasurface-based biosensors consisting of gold nano antenna arrays loaded with graphene and working in the mid infrared (mid-IR) spectral range can achieve simultaneous high-sensitivity and high-specificity detection of biomolecules. Strong light molecule interactions in deeply subwavelength optical spots created by the biosensors allow us to determine the concentration of protein molecules via spectral shifts of the metasurface resonance. A combination of passive and active tuning of the metasurface sensors allows us to spectrally overlap the metasurface resonance and the protein vibrational modes, so that protein molecules can be identified via their characteristic mid-IR fingerprints. The high sensitivity and specificity of the metasurface sensors enable us to determine the secondary structure of protein immunoglobulin (IgG) molecules 4 orders of magnitude more sensitive than attenuated total reflection Fourier transform infrared spectroscopy.