期刊
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
卷 7, 期 4, 页码 3715-3721出版社
AMER CHEMICAL SOC
DOI: 10.1021/acssuschemeng.8b06210
关键词
Online optical detection platform; Brightfield microscope; Visible light absorption; Ascorbate; In vivo
资金
- National Natural Science Foundation [21575090]
- High-level Teachers in Beijing Municipal Universities in the Period of 13th Five-year Plan [CITTCD20190330]
- Scientific Research Project of Beijing Educational Committee [KM201810028008]
- Youth Innovative Research Team of Capital Normal University
- Capacity Building for Sci-Tech Innovation -Fundamental Scientific Research Funds [025185305000/195]
Online electrochemical systems (OECSs) possess a distinct continuously monitoring ability toward electro-active species and have been intensively used for single/multiple neurochemical measurements. However, detecting electro-inactive species or some substances being oxidized/reduced at close potentials is difficult in OECS, and it is highly demanded to broaden such useful online methodology to more new principles, which, however, is still lacking. The system reported in this Letter demonstrates a solution to the aforementioned problem. We report an online optical detection platform (OODP) based on visible light absorption for the continuous monitoring of neurochemicals in rat brains with ascorbic acid (AA) as an example. The OODP is constructed with a homemade online capillary-based microfluidic line as the optical detector built under a brightfield microscope. Cobalt oxyhydroxide (CoOOH) nanoflakes directly oxidizing 3,3',5,5'-tetramethylbenzidine (TMB) into a blue-colored product (oxTMB) which can be faded by AA paves a principle for selective AA recognition. Combined with in vivo microdialysis sampling, an in vivo AA monitoring assay was established by directly integrating this visible light absorption-based probe and the brightfield microscope-based OODP. The OODP for AA measurement possesses excellent stability, reproducibility, a wide linear detection range from 0.5 to 100 mu M, and can successfully record the cerebral AA alterations in the clam/ischemia model.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据