期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 55, 期 13, 页码 4236-4241出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201511868
关键词
hydroxyl radicals; living cells; luminescent probes; quantum dots; real-time monitoring
资金
- National Basic Research Program of China (973 Program) [2014CB932103]
- National Natural Science Foundation of China [21575010, 21375006]
- Innovation and Promotion Project of the Beijing University of Chemical Technology
The utilization of semiconductor quantum dots (QDs) as optical labels for biosensing and biorecognition has made substantial progress. However, the development of a suitable QD-based luminescent probe that is capable of detecting individual reactive oxygen species (ROS) represents a great challenge, mainly because the fluorescence of QDs is quenched by a wide variety of ROS. To overcome this limitation, a novel QD-based turn-on luminescent probe for the specific detection of (OH)-O-. has been designed, and its application in monitoring the endogenous release of (OH)-O-. species in living cells is demonstrated. Metal citrate complexes on the surfaces of the QDs can act as electron donors, injecting electrons into the LUMO of the QDs, while (OH)-O-. can inject holes into the HOMO of the QDs. Accordingly, electron-hole pairs are produced, which could emit strong luminescence by electron-hole recombination. Importantly, this luminescent probe does not respond to other ROS.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据