期刊
JOURNAL OF PARKINSONS DISEASE
卷 9, 期 2, 页码 315-326出版社
IOS PRESS
DOI: 10.3233/JPD-191590
关键词
alpha-Synuclein; Parkinson's disease; engrailed; proteinopathy; misfolded proteins; propagation; mouse models; neurodegeneration
资金
- Van Andel Research Institute
- Jose Castillejo Mobility Grant - Ministry of Education, Culture and Sport (Madrid, Spain) [CAS15/00201]
- Peter C. and Emajean Cook Foundation
- National Institutes of Health [1R01DC016519-01, 5R21NS093993-02, R21NS101676, R01NS060729]
- Rush University center grant from the Parkinson's Disease Foundation
- NIH IGNITE grant [1R21NS106078-01A1]
Background: Parkinson's disease (PD) is a synucleinopathy that has multiple neuropathological characteristics, with nigrostriatal dopamine system degeneration being a core feature. Current models of PD pathology typically fail to recapitulate several attributes of the pathogenic process and neuropathology. We aimed to define the effects of combining a mouse model exhibiting multiple PD-like changes with intrastriatal injections of alpha-synuclein (alpha-syn) pre-formed fibril (PFFs) aggregates. We employed the heterozygous Engrailed 1 (En1(+/-)) mouse that features several pathophysiological hallmarks of clinical PD. Objective: To test the hypothesis that the neuropathological changes in the En1(+/-) mice will promote formation of alpha-syn aggregates following intrastriatal injections of pathogenic human alpha-syn PFFs. Methods: We unilaterally injected PFFs into the striata of 1-month-old En1(+/-) and control wild-type mice and euthanized animals at 3 months for post-mortem analysis. Results: Using immunohistochemistry and unbiased stereology, we established that PFF-injected En1(+/-) mice exhibited a near-threefold increase in pS129-alpha-syn-positive neurons in the substantia nigra compared to PFF-injected wild-type mice. The PFF-injected En1(+/-) mice also displayed significant increases in pS129-alpha-syn-positive neurons in the amygdala and ventral tegmental area; regions of known PD pathology with projections to the striatum. Additionally, we observed amplified pS129-alpha-syn-positive aggregation in En1(+/-) mice in multiple cortical regions. Conclusions: Following intrastriatal injection of PFFs, absence of an En1 allele leads to additional aggregation of pathological alpha-syn, potentially due to En1-loss mediated nigrostriatal impairment. We propose that further development of this double-hit model could result in a PD mouse model that predicts which experimental therapies will be effective in PD.
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