期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 55, 期 47, 页码 14728-14732出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201609895
关键词
analytical methods; fluorescent probes; reactive oxygen species; recognition moiety; tyrosinase
资金
- 973 Program [2015CB932001, 2015CB856301]
- NSF of China [21535009, 21675159, 21435007, 21321003]
- Chinese Academy of Science [XDB14030102]
Fluorescence imaging of tyrosinase (a cancer biomarker) in living organisms is of great importance for biological studies. However, selective detection of tyrosinase remains a great challenge because current fluorescent probes that contain the 4-hydroxyphenyl moiety show similar fluorescence responses to both tyrosinase and some reactive oxygen species (ROS), thereby suffering from ROS interference. Herein, a new tyrosinase-recognition 3-hydroxybenzyloxy moiety, which exhibits distinct fluorescence responses for tyrosinase and ROS, is proposed. Using the recognition moiety, we develop a near-infrared fluorescence probe for tyrosinase activity, which effectively eliminates the interference from ROS. The high specificity of the probe was demonstrated by imaging and detecting endogenous tyrosinase activity in live cells and zebrafish and further validated by an enzyme-linked immunosorbent assay. The probe is expected to be useful for the accurate detection of tyrosinase in complex biosystems.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据