期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 55, 期 26, 页码 7483-7486出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201603183
关键词
DNA origami; enzymes; NADH; self-assembly; substrate channeling
资金
- DOD-Navy-ONR MURI [W911NF-12-1-0420]
- Army Research Office [W911NF-11-1-0137]
- National Science Foundation [14030615]
- National Science Foundation of China [21325522, 21422506, 21275122, 21521004]
- Program for Changjiang Scholars and Innovative Research Team in University [IRT13036]
- China Scholarship Council
Artificial multi-enzyme systems with precise and dynamic control over the enzyme pathway activity are of great significance in bionanotechnology and synthetic biology. Herein, we exploit a spatially addressable DNA nanoplatform for the directional regulation of two enzyme pathways (G6pDH-MDH and G6pDH-LDH) through the control of NAD(+) substrate channeling by specifically shifting NAD(+) between the two enzyme pairs. We believe that this concept will be useful for the design of regulatory biological circuits for synthetic biology and biomedicine.
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