期刊
FORESTS
卷 10, 期 3, 页码 -出版社
MDPI
DOI: 10.3390/f10030214
关键词
Populus tomentosa; AhDREB; salt; unintended effects; photosynthesis; antioxidant defense system
类别
资金
- Science and Technology Development Center of the State Forestry Administration Project [JC-2017-02]
- National Science and Technology Major Project of the Ministry of Science and Technology of China [2018ZX08020002-003-002]
- Fundamental Research Funds for the Central Universities [2016BLPX13]
- National High Technology Research and Development Program of China [2013AA102703]
The DREB transcription factors regulate multiple stress response genes, and are therefore useful for molecular plant breeding. AhDREB, a stress-inducible gene, was isolated from Atriplex hortensis L. and introduced into Populus tomentosa Carriere under the control of the CaMV35S promoter. Under salt stress, the chlorophyll content and net photosynthetic rate were higher in transgenic lines than in the wild type (WT). Moreover, the rate of electrolyte penetration (REC) was lower in the transgenic lines. Additional analyses revealed that the AhDREB transgenic plants generally displayed lower malondialdehyde (MDA) activity but higher superoxide dismutase (SOD) and peroxidase (POD) activities and proline content than the WT under salt stress. RNA sequencing indicated that AhDREB could enhance tolerance to salt by activating various downstream genes in the transgenic plants. Furthermore, no growth inhibition was detected in transgenic plants expressing AhDREB driven by the constitutive CaMV35S promoter. The transcriptome showed 165 and 52 differentially expressed genes in transgenic plants under stress and non-stress conditions, respectively, among which no significant metabolic pathway was enriched and no unintended effects have yet been identified. Together, these results suggest that AhDREB may be a good candidate gene for increasing salt tolerance in transgenic poplar breeding.
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