期刊
CELL REPORTS
卷 26, 期 13, 页码 3762-+出版社
CELL PRESS
DOI: 10.1016/j.celrep.2019.02.100
关键词
-
类别
资金
- Chemestmed Ltd.
- Centre of Excellence in Molecular Cell Engineering, Estonia [2014-2020.4.01.15-013]
- Estonian Research Council [PUT582]
- Finnish Foundation for Cardiovascular Research (Syda ntutkimussaatio)
Chemical modifications of RNA provide an additional, epitranscriptomic, level of control over cellular functions. N-6-methylated adenosines (m6As) are found in several types of RNA, and their amounts are regulated by methyltransferases and demethylases. One of the most important enzymes catalyzing generation of m6A on mRNA is the trimer N-6-methyltransferase METTL3-14-WTAP complex. Its activity has been linked to such critical biological processes as cell differentiation, proliferation, and death. We used in silico-based discovery to identify small-molecule ligands that bind to METTL3-14-WTAP and determined experimentally their binding affinity and kinetics, as well as their effect on enzymatic function. We show that these ligands serve as activators of the METTL3-14-WTAP complex.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据