期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 55, 期 41, 页码 12727-12730出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201605833
关键词
chirality; fluorescent probes; oxidoreductases; photophysics; redox chemistry
Methionine sulfoxide reductaseA (MsrA) is an enzyme involved in redox balance and signaling, and its aberrant activity is implicated in a number of diseases (for example, Alzheimer's disease and cancer). Since there is no simple small molecule tool to monitor MsrA activity in real time invivo, we aimed at developing one. We have designed a BODIPY-based probe called (S)-Sulfox-1, which is equipped with a reactive sulfoxide moiety. Upon reduction with a model MsrA (E. coli), it exhibits a bathochromic shift in the fluorescence maximum. This feature was utilized for the real-time ratiometric fluorescent imaging of MsrA activity in E. coli cells. Significantly, our probe is capable of capturing natural variations of the enzyme activity invivo.
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