期刊
CELL REPORTS
卷 26, 期 5, 页码 1213-+出版社
CELL PRESS
DOI: 10.1016/j.celrep.2018.12.106
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资金
- Ministry of Education, Culture, Sports, Science and Technology in Japan (MEXT)/Japan Society for the Promotion of Science (JSPS) KAKENHI [26460396, 17K08547, 17K09845, 16K19545, 15H05723, 16H06536, 15H04272]
- Japan diabetes foundation
- Kyorin University School of Medicine Joint research program
- Gunma University Joint research program [15010]
- JST CREST, Japan [JPMJCR1751]
- program for Brain Mapping by Integrated Neurotechnologies for Disease Studies (Brain/MINDS) from MEXT
- Japan Agency for Medical Research and Development (AMED)
- Novo Nordisk Pharma
- Takeda Science Foundation
- Science Research Promotion Fund from the Promotion and Mutual Aid Corporation for Private Schools of Japan
- Grants-in-Aid for Scientific Research [17K09845, 16K19545, 17K08547, 15H05723, 15H04272] Funding Source: KAKEN
Pancreatic beta cells secrete insulin by Ca2+-triggered exocytosis. However, there is no apparent secretory site similar to the neuronal active zones, and the cellular and molecular localization mechanism underlying polarized exocytosis remains elusive. Here, we report that ELKS, a vertebrate active zone protein, is used in beta cells to regulate Ca2+ influx for insulin secretion. beta cell-specific ELKS-knockout (KO) mice showed impaired glucose-stimulated first-phase insulin secretion and reduced L-type voltage-dependent Ca2+ channel (VDCC) current density. In situ Ca2+ imaging of beta cells within islets expressing a membrane-bound G-CaMP8b Ca2+ sensor demonstrated initial local Ca2+ signals at the ELKS-localized vascular side of the beta cell plasma membrane, which were markedly decreased in ELKS-KO beta cells. Mechanistically, ELKS directly interacted with the VDCC-beta subunit via the GK domain. These findings suggest that ELKS and VDCCs form a potent insulin secretion complex at the vascular side of the beta cell plasma membrane for polarized Ca2+ influx and first-phase insulin secretion from pancreatic islets.
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