期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 55, 期 21, 页码 6285-6289出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201600590
关键词
enzyme catalysis; nanobiocatalysts; nanoparticles; organosilica; self-assembly
资金
- Swiss Nanoscience Institute (SNI) through the NanoArgovia program (NanoZyme project)
- Swiss Commission for Technology and Innovation [16437.1 PFEN-NM]
- EU-Eurostars program [E!6894]
- Swiss State Secretariat for Education, Research and Innovation (EU INMARE project)
The fragile nature of most enzymes is a major hindrance to their use in industrial processes. Herein, we describe a synthetic chemical strategy to produce hybrid organic/inorganic nanobiocatalysts; it exploits the self-assembly of silane building blocks at the surface of enzymes to grow an organosilica layer, of controlled thickness, that fully shields the enzyme. Remarkably, the enzyme triggers a rearrangement of this organosilica layer into a significantly soft structure. We demonstrate that this change in stiffness correlates with the biocatalytic turnover rate, and that the organosilica layer shields the enzyme in a soft environment with a markedly enhanced resistance to denaturing stresses.
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