期刊
SCIENTIFIC REPORTS
卷 9, 期 -, 页码 -出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/s41598-019-39175-5
关键词
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资金
- National Research Foundation of Korea (NRF) - Korea government (MSIP) [2016R1C1B1014991]
- Korean Health Technology R&D Project, Ministry of Health & Welfare, Republic of Korea [HI15C1512]
- National Research Foundation of Korea [2016R1C1B1014991] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Isolation and concentration of fungi in the blood improves sensitivity of the polymerase chain reaction (PCR) method to detect fungi in blood. This study demonstrates a sheathless, continuous separation and concentration method of candida cells using a viscoelastic fluid that enables rapid detection of rare candida cells by PCR analysis. To validate device performance using a viscoelastic fluid, flow characteristics of 2 mu m particles were estimated at different flow rates. Additionally, a mixture of 2 mu m and 13 mu m particles was successfully separated based on size difference at 100 mu l/min. Candida cells were successfully separated from the white blood cells (WBCs) with a separation efficiency of 99.1% and concentrated approximately 9.9-fold at the center outlet compared to the initial concentration (similar to 2.5 x 10(7) cells/ml). Sequential 1st and 2nd concentration processes were used to increase the final number of candida cells to similar to 2.3 x 10(9) cells/ml, which was concentrated similar to 92-fold. Finally, despite the undetectable initial concentration of 10(1) CFU/ml, removal of WBCs and the additional buffer solution enabled the quantitative reverse transcription (RT)-PCR detection of candida cells after the 1st concentration (Ct = 31.43) and the 2nd concentration process (Ct = 29.30).
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