期刊
NUTRIENTS
卷 11, 期 2, 页码 -出版社
MDPI
DOI: 10.3390/nu11020404
关键词
(E)-5-hydroxy-7-methoxy-3-(2-hydroxybenzyl)-4-chromanone; Portulaca oleracea; INS-1 pancreatic cell; glucotoxicity
资金
- National Research Foundation of Korea (NRF) [2017R1A2B4005323]
- National Research Foundation of Korea [2017R1A2B4005323] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
In this study, we investigated whether (E)-5-hydroxy-7-methoxy-3-(2-hydroxybenzyl)-4-chromanone, a homoisoflavonoid compound isolated from Portulaca oleracea L., protects INS-1 pancreatic cells against glucotoxicity-induced apoptosis. Treatment with high glucose (30 mM) induced apoptosis in INS-1 pancreatic cells; however, the level of cell viability was significantly increased by treatment with (E)-5-hydroxy-7-methoxy-3-(2-hydroxybenzyl)-4-chromanone. Treatment with 10-20 mu M of (E)-5-hydroxy-7-methoxy-3-(2-hydroxybenzyl)-4-chromanone dose-dependently increased cell viability and significantly decreased the intracellular level of reactive oxygen species (ROS), thiobarbituric acid reactive substances (TBARS), and nitric oxide levels in INS-1 pancreatic cells pretreated with high glucose. These effects were associated with increased anti-apoptotic Bcl-2 protein expression, while reducing pro-apoptotic Bax, cytochrome C, and caspase 9 protein expression. Treatment with (E)-5-hydroxy-7-methoxy-3-(2-hydroxybenzyl)-4-chromanone reduced the apoptosis previously induced by high-level glucose-treatment, according to annexin V/propidium iodide staining. These results demonstrate that (E)-5-hydroxy-7-methoxy-3-(2-hydroxybenzyl)-4-chromanone may be useful as a potential therapeutic agent to protect INS-1 pancreatic cells against high glucose-induced apoptosis.
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